Chromatography Essay Topics

Separation of Photosynthetic Pigments by Paer Chromatography

I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment exists in two forms; chlorophyll a and chlorophyll b. Chlorophyll a, being the main photosynthetic pigment, has a primary purpose to convert light energy to chemical energy used by the… View Article

Chromatography of M&M and Ink Dyes

Separations: Chromatography of M&M and Ink Dyes Almost all substances we come into contact with on a daily basis are impure; that is, they are mixtures. Similarly, compounds synthesized in the chemical laboratory are rarely produced pure. As a result, a major focus of research in chemistry is designing methods of separating and identifying components… View Article

High performance

Conclusion: There are 3 different component dyes in the mix including blue dye, yellow dye and red dye. The blue dye has the highest Rf in chromatography ( Rf=1 in water and ethanol and isoprophy alcohol). The yellow dye has the lowest Rf in chromatography in ethanol and isoprophy alcohol and middle Rf in water… View Article

Plant Pigment Chromatography

1. Describe what each of your chromatography strips looked like. Specifically, identify the pigments on each strip and compare their positions to one another. Plants have four types of pigments, namely chlorophyll, carotenoids, anthocyanins, and xanthophylls. These pigments have different polarities and chemical properties. In paper chromatography, the pigments will separate based on their affinity… View Article

Lab report for chromatography

Study Questions: One of the requirements for the photosynthesis process to occur, the plant cells need sufficient light. Pigments are the substances that absorbed visible light. Different pigments absorb light of different wavelengths. From the absorption spectrum of chlorophyll a, it suggests that blue and red light work best for photosynthesis. There is a high… View Article

Steam Distillation of Eugenol

Introduction: This experiment involves an extraction of a natural product using the techniques of steam distillation. The principle component of oil of cloves is an aromatic compound, which is identified by thin layer chromatography (TLC). Eugenol is widely used in dentistry, due to its analgesic, antiseptic balsamic qualities. It is ideal for curative for pulp… View Article

HPLC Analysis of Caffeine

The main objective of this lab is to gain experience in using and reading results from the HPLC machine. 2) The first step is to inject a series of caffeine standards into the machine in order to get results/values of peak height and area. 3) The next step is to investigate the effects of a… View Article

Column and Thin layer Chromatography

ABSTRACT Chromatography is the separation of mixtures into their constituents. It relies on the differential solubilities or absorptivities of the components to be separated with respect to two phases, one of which is stationary and the other, mobile. The group aims to separate the coloured components of siling labuyo using column chromatography then determine the… View Article

Fractional Distillations

In this experiment we aim to demonstrate that we can separate two volatile compounds from a mixture due to the different chemical properties of each compound. We will accomplish this by a separation procedure known as distillation, which relies on each compound having a distinct and separate boiling point. Our pure products will be analyzed… View Article

Column and thin layer chromatography

Chromatography is a technique used to separate mixtures into components as a result of differential partitioning behavior between a stationary phase and a mobile phase that percolates through the stationary bed. [1, 2] Column and thin layer chromatography are examples of the solid-liquid method of chromatography, which utilizes an adsorbent solid stationary phase (silica) and… View Article

Difference in Lymphatic Function in Health and Disease State

ABSTRACT High Performance Liquid Chromatography has been used to evolve an analytical procedure for the evaluation of the content of paracetamol in the bulk, dosage forms and in urine, a body fluid. Separation and resolution have been achieved with a combination of methanol and 2.5% acetic acid (15:85) on a reversed-phase column at ambient temperature…. View Article


Codeine or methyl morphine, an alkaloid, was first isolated in 1832 from raw opium. It concentration ranges from 0.2% to 0.8%. Mostly used for its analgesic, anti-tussive and anti-diarrheal capabilities (Tremlett, Anderson and Wolf, 2010). Paracetamol also known as acetaminophen (n-acetyl-p-aminophenol, APAP) on the other hand, is a useful non- steroidal anti- inflammatory drug (NSAID)…. View Article